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KMID : 0903520090520010070
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 1 p.70 ~ p.75
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Production of Reactive Oxygen Species and Changes in Antioxidant Enzyme Activities during Differentiation of 3T3-L1 Adipocyte
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Lee Ok-Hwan
Kwon Young-In
Hong Hee-Do
Park Cheon-Seok
Lee Boo-Yong
Kim Young-Cheul
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Abstract
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Obesity, a major public health problem around the world, is a strong risk factor for the development of type 2 diabetes, atherosclerosis, hypertension and cardiovascular diseases. Recent research suggests that increased production of reactive oxygen species (ROS) from accumulated fat in obesity leads to elevated systemic oxidative stress and contributes to the development of obesitylinked chronic disorders. The aim of the current study was to investigate changes of key enzymes associated with antioxidant response and metabolic pathways in 3T3-L1 cell, a preadipocyte cell line that undergoes differentiation into mature adipocytes. The changes in lipid accumulation, ROS production, glucose-6-phosphate dehydrogenase (G6PDH), superoxide dismutase (SOD) and catalase (CAT) during the course of differentiation were determined. The ROS production and G6PDH activity exponentially increased as differentiation progressed. However, CAT activity showed a sharp decrease until day 2, followed by a gradual increase up to day 6 and then leveled off. Given the importance of adipocyte differentiation and ROS production in oxidation-linked diseases, these data provide a biochemical rationale for further studies to identify cellular mechanisms that can counter ROS generation and modulate cellular redox environment in adipocytes.
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KEYWORD
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antioxidant enzyme response, glucose-6-phosphate dehydrogenase, hexose monophosphate shunt, reactive oxygen species, 3T3-L1 adipocyte differentiation
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